Quantifying Double-Strand Breaks and Clustered Damages in DNA by Single-Molecule Laser Fluorescence Sizing

Quantifying double-strand breaks and clustered damages in DNA by single-molecule laser fluorescence sizing.

Fluorescence from a single DNA molecule passing through a laser beam is proportional to the size (contour length) of the molecule, and molecules of different sizes can be counted with equal efficiencies. Single-molecule fluorescence can thus determine the average length of the molecules in a sample and hence the frequency of double-strand breaks induced by various treatments. Ionizing radiation...

UVA-induced DNA double-strand breaks result from the repair of clustered oxidative DNA damages

UVA (320-400 nm) represents the main spectral component of solar UV radiation, induces pre-mutagenic DNA lesions and is classified as Class I carcinogen. Recently, discussion arose whether UVA induces DNA double-strand breaks (dsbs). Only few reports link the induction of dsbs to UVA exposure and the underlying mechanisms are poorly understood. Using the Comet-assay and γH2AX as markers for dsb...

DNA Double-Strand Breaks

The activation-induced cytidine deaminase (AID) is required for somatic hypermutation (SHM) and class-switch recombination (CSR) of immunoglobulin (Ig) genes, both of which are associated with DNA double-strand breaks (DSBs). As AID is capable of deaminating deoxy-cytidine (dC) to deoxy-uracil (dU), it might induce nicks (single strand DNA breaks) and also DNA DSBs via a U-DNA glycosylase-media...

Simulation of strand breaks induced in DNA molecule by radiation of proton and Secondary particles using Geant4 code

Radiotherapy using various beams is one of the methods for treating cancer, Hadrons  used   to  treat cancers  that  are  near critical organs. The most important part of the cell that is damage by ionizing radiation is DNA. In this study, damages induced in the  genetic material of  living cells (DNA) defined by  the  atomic model from the  protein data bank (PDB) have been studied by  radiati...

DNA Double-Strand Breaks Relieve